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. 2017 Jul 17;7:5586. doi: 10.1038/s41598-017-05839-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Laser confocal microscopy images of sepiolite fibers (0, 5 and 50 ng·μl⁻¹) in V79 cells. (A) Channels: IR (infrared), red, green and blue, at given excitation and emission wavelengths. Sepiolite has a natural fluorescence in the green and red ranges and is not fluorescent in blue or infrared. The blue fluorescence represents the Dako-DAPI staining of the cell nuclei. In merged images, we confirmed that sepiolite fibers were uptaken by the cells. IR: infrared. The sepiolite concentration is indicated on the figure. The wavelengths of excitation (Exc) and fluorescence emission (Emis) are indicated. (B) Magnified fluorescence microscopy images of sepiolite fibers in V79 cells. Nucleus in blue (DAPI staining), sepiolite fibers in orange/red. (C) Phase contrast in confocal analysis visualizing cell contour and the presence of sepiolite (green) inside the cell.