Figure 5.

Time kinetics for sepiolite versus Sep/DNA interaction with V79 cells. 100 μL of sepiolite dispersion was prepared as following: 45 μL of sepiolite at 2 mg·mL⁻¹ and 55 μl of 10 mM TrisHCl pH 7.5. 8.9 ml of cellular medium (MEM) was added and gently homogenized. Finally, 3 mL of sepiolite at 10 ng·μL⁻¹ in cellular medium was added in each set of 3 wells with 2x10⁴ of V-79 cells in each well. Cells were collected after trypsinization and analysed by FACS 1 h, 4 h, 6 h, and 24 h after addition of sepiolite. 10,000 cells were counted for each sample.