Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 17;7:5522. doi: 10.1038/s41598-017-05816-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

AC9 activity and AKAP association in heart. (A,B) Membranes were prepared from 6 wk WT versus AC9^−/− heart. (A) AC activity was measured under basal conditions and upon stimulation with 300 nM Gαs or 50 µM forskolin (n = 4, performed in duplicate or triplicate). (B) AC activity was measured in the presence of increasing concentrations of SQ22,536 in the presence of 300 nM Gαs (n = 3, performed in duplicate). (C,D) Heart extracts from WT or AC9^−/− mice were subjected to immunoprecipitation (IP) with pre-immune (control) or anti-Yotiao (C) and control IgG or anti-AKAP5 (D). AKAP-associated AC activity was stimulated with 300 nM Gαs and measured (n = 3–4). Data are shown as mean +/− SD. A portion of the IP’s from (C and D) were subjected to WB analysis for the appropriate AKAP. (E) Yotiao-associated AC activity was measured from WT sinoatrial node (SAN), left ventricle (LV), and right atrium (RA) tissue homogenates as in panel (C). Immunoblot of Yotiao from IPs is shown below. (F) Yotiao-associated activity from WT and AC9^−/− SAN as measured in panel (C); Yotiao immunoblot is shown below (n = 3). Full-length WBs are presented in Supplementary Fig. S6.