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. 2017 Jul 17;7:5584. doi: 10.1038/s41598-017-05971-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The blockage of RNAi in B. dorsalis can be induced by injecting hemolymph collected from dsRNA-challenged flies. (A) mRNA accumulation of the target gene after feeding ds-rpl19 followed by injection of Ch hemolymph which has been incubated at 25 °C for 24 h, 48 h and 72 h. (B) mRNA accumulation of the target gene after feeding ds-rpl19 followed by injecting hemolymph treated at different temperatures. Normalized target gene mRNA accumulation is reported relative to mRNA accumulation of the ds-egfp control, which was set to 1. All error bars represent the S.E. of the mean of three independent biological replicates. * indicates a statistically significant difference in rpl19 mRNA accumulation between the feeding ds-rpl19 group and the control ds-egfp group (P < 0.05, Student’s t-test). Different letters indicate a significant difference in rpl19 mRNA accumulation among the feeding ds-rpl19 treatments (P < 0.05, Duncan’s test).