Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 17;7:5584. doi: 10.1038/s41598-017-05971-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The blockage of RNAi caused by injecting linoleic acid. (A) mRNA accumulation of the target gene after feeding ds-rpl19 followed by injection of LA at different concentrations. (B) Feeding with dsRNA at 24 h, 48 h and 72 h after injecting LA, the mRNA accumulation of the rpl19. (C) mRNA accumulation levels of genes known to be required for the endocytic entry of dsRNAs after injecting LA. (D) Subcellular localization of Cy3-labelled ds-rpl19 in midgut tissue after the injection of LA into B. dorsalis. The scale bar represents 25 μm. Normalized target gene mRNA accumulation is reported relative to mRNA accumulation of the ds-egfp control, which was set to 1. All error bars represent the S.E. of the mean of three independent biological replicates. * indicates a statistically significant difference in rpl19 mRNA accumulation between the feeding ds-rpl19 group and the control ds-egfp group (P < 0.05). ** indicates P < 0.01 (Student’s t-test). Different letters indicate a significant difference in rpl19 mRNA accumulation among the feeding ds-rpl19 treatments (P < 0.05, Duncan’s test).