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. 2017 Jul 17;7:5584. doi: 10.1038/s41598-017-05971-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The influence of AA injection on the RNAi effect induced by the feeding of dsRNA in D. melanogaster. (A) mRNA accumulation of the target gene after feeding ds-Dmrpl19 followed by injecting AA. (B) Subcellular localization of Cy3-labelled ds-Dmrpl19 in midgut tissue of D. melanogaster after the injection of AA. The scale bar represents 25 μm. Normalized target gene mRNA accumulation is reported relative to mRNA accumulation in the ds-egfp control, which was set to 1. All error bars represent the S.E. of the mean of three independent biological replicates. * indicates a statistically significant difference in rpl19 mRNA accumulation between the feeding ds-rpl19 group and the control ds-egfp group (P < 0.05, Student’s t-test).