Table 3.

Validation of highly upregulated or downregulated genes using real-time quantitative PCR.

Gene	RT-qPCR		RNA-Seq
	Fold change (FF vs NF)	p value	Fold change (FF vs NF)	p value
MYOCD	+30.22**	0.005	+11.45**	0.006
LMO3	+7.11*	0.046	+9.13**	0.003
IL-6	+2.70**	0.001	+3.04***	0.0003
RELB	+1.74*	0.017	+2.14**	0.001
PRG4	−47.62*	0.023	−49.61***	0.0006
CD34	−28.57*	0.033	−14.75**	0.003
IL-33	−27.78*	0.043	−19.90***	0.0005
MMP-10	−20.06*	0.018	−16.54***	0.0003
WISP2	−15.15*	0.027	−8.78**	0.009
COL6A6	−13.39*	0.033	−7.41***	0.0008
IGFBP5	−6.29*	0.022	−3.94**	0.002

All mRNA values were normalised relative to that of GAPDH and triplicate experiments were performed for each gene. Statistically significant differences were expressed as *p < 0.05; **p < 0.01; ***p < 0.001.