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. 2017 Jul 17;7:5575. doi: 10.1038/s41598-017-06008-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sca-1⁺ and Epcam⁺ cells isolated from mouse placentae at E11.5 expressed the trophoblast marker, Krt8 by qRT-PCR. These cells also expressed other known markers of trophoblast, including Cdx2, Eomes and Epcam. Epcam was expressed in both Sca-1⁺ and Epcam⁺ cells however Sca-1 was only detected in Sca-1⁺ cells. The syncytiotrophoblast marker, Gcm1, was detected only in Epcam⁺ cells.