Figure 3.

PGI₂ reverses stress fibre formation independent of ADP and TXA₂. Platelets (2 × 10⁷/ml) were spread on 100 μg/ml fibrinogen for 25 minutes with or without apyrase (2 U/ml) and indomethacin (10 μM). The platelets were washed, and 10 nM PGI₂ added for 10 minutes, in the presence or absence of apyrase (2 U/ml) and indomethacin (10 μM). The platelets were then fixed and stained with FITC-phalloidin before being imaged. (a) Images are representative of the experimental conditions. (b) The number of platelets containing stress fibres or actin nodules in the presence or absence of apyrase (2 U/ml) and indomethacin (10 μM) was identified in control and PGI₂ treated samples. (c) The total number of platelets adhered, was identified in control and PGI₂ treated samples. (d) The average surface area of the spread platelets was identified in control and PGI₂ treated samples. The experiments are an average of n = 4. Scale bar is 5 μm. p < 0.05.