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. 2017 Jul 17;7:5645. doi: 10.1038/s41598-017-05944-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) The stability of PBAF subunits in human cells. HEK293 were treated with 20 µg/ml cycloheximide (CHX) for the time points indicated above the panels. The equal amounts of lysates of control (0 h) and CHX-treated HEK293 were then analyzed by Western blotting. β-Actin was used as loading control. (B) The intensity of bands on Western blot was quantified using ImageJ software by densitometry as described in Methods section. The images in Fig. 1(A) is cropped.