Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 17;7:5574. doi: 10.1038/s41598-017-05768-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

TRIB1 and HNF4A form a complex. (A), Constructs coding for myc-tagged BirA alone or inserted N terminal of TRIB1 were co-transfected with HNF4A expression plasmids in HEK293T for 24 h. Following an additional 24 h growth in media supplemented with 0.1 mM biotin, cells were harvested and lysed under denaturing conditions. Biotinylated targets were then isolated using streptavidin beads and analyzed by Western blotting using cognate antibodies followed by streptavidin coupled to IRDye800CW. (B), Plasmids encoding either HNF4A or HNF1A were transfected alongside FLAG-tagged TRIB1 plasmids in HEK293T cells. Potential complexes were isolated with FLAG-specific immunobeads and analyzed by Western blotting. (C), Interaction is independent of DNA binding. Ethidium bromide (0.2 mg/ml) was included during the immunoprecipitation. Samples were analyzed by Western blotting using the indicated antibodies.