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. 2017 Jul 17;7:5610. doi: 10.1038/s41598-017-06024-2

Figure 5.

Figure 5

S. aureus-induced platelet cell death. (a) MFI of platelet-bound DIOC6(3) (b) Annexin V binding (c) PMP concentration or (d) fold increase in active caspase-3 expression were analyzed by flow cytometry after 30 min of exposure to Tyrode’s buffer (NS), TRAP, staphylococcal strains [clinical strains (SaB), S. condimenti (Sc) or S. aureus ATCC 43866 (ATCC43866)] or the A23187 apoptosis inducer as a positive control. The data (mean ± SEM; n = 8 experiments) are expressed as the AU (a), % expression (b) or fold increase compared with NS (d). The PMP concentration (mean ± SEM; n = 6–27 experiments) (c) is expressed as microparticles/µl. *p < 0.05, **p < 0.01, ****p < 0.0001 (one-way ANOVA with repeated measures and the Bonferroni post hoc test; stimulated vs. unstimulated, NS).