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. Author manuscript; available in PMC: 2017 Jul 18.
Published in final edited form as: Arthritis Rheumatol. 2016 May;68(5):1301–1313. doi: 10.1002/art.39538

Figure 1.

Figure 1

RANKL induced CD97 expression during osteoclastogenesis. A) TN c-fms+ cells from WT mice were cultured for 4 days with M-CSF in the absence or presence of RANKL before RT-PCR was performed. B) Flow cytometric analysis of bone marrow cells indicates the expression of CD97 in osteoclast precursor cells (~3.2 %). C) BMM cells were cultured for up to 6 days either with M-CSF or M-CSF and RANKL. RNA was converted to cDNA and real-time PCR was performed D) CD97 protein expression was transiently detected in RANKL treated BMM cultures at day 2 and peaked at day 3. Values represent mean±SEM. *, Significant effect of RANKL treatment, p<0.05. TN: triple negative, B220−CD3−CD11b−/lo.