Fig. 1.

TC-1 tumor growth inhibition by vaccine delivery in vivo. a TC-1 tumor growth inhibition in mice by lipid/E7 complexes. Six-week-old female C57BL/6 mice were inoculated with 10,000 TC-1 cells s.c. in the abdomen on day zero. On day 6, treatments were s.c. injected into the opposite side of the abdomen. Lipid formulations delivered contained 100 nmol lipid and 20 μg of E7 peptide. Sample size and statistics are as follows: n = 5–6, *: P < 0.05, **: P < 0.01, ***: P < 0.0001. b Dose-dependent antitumor activity of racemic DOTAP/E7, (R)-DOTAP/E7, or (S)-DOTAP/E7 complexes. Six-week-old female C57BL/6 mice were injected with 10,000 TC-1 cells s.c. as in previous studies. On day 6, treatments were s.c. injected into the opposite side of the abdomen. The dose of lipid was varied while the dose of E7 peptide was kept constant at 20 nmol. n = 5–6 per dose