Fig. 3.

IFN-γ production from splenocytes of (R)-DOTAP/E7- or (S)-DOTAP/E7-treated mice. a Splenocytes isolated (on day 14) from tumor-bearing mice treated with (R) or (S)-DOTAP/E7 at 300 nmol/20 nmol (on day 6). Splenocytes were pulsed with E7 or an irrelevant peptide (NP) for 6 h, washed, and stained for CD8 and IFN-γ. The numbers on the contour plots indicate percentage of IFN-γ⁺ CD8⁺ T cells out of all CD8⁺ T cells. One representative experiment is shown. b IFN-γ-secreting CD8⁺ cells per 10⁴ CD8⁺ T cells after 6 h pulse with peptide. n = 6–8. **: P < 0.01 c IFN-γ production after 12 h pulse with either NP or E7 peptide. d IFN-γ-secreting CD8⁺ cells per 10⁴ CD8⁺ T cells after 12 h pulse with peptide. n = 6-8. *: P < 0.02