Fig. 4.

In vivo cytotoxic T-lymphocyte (CTL) assay. a Targets pulsed with E7 or an irrelevant peptide (NP) were stained with high (E7) or low (NP) concentrations of CFSE, injected into treated mice and in vivo killing was allowed for 16-20 h, then spleens removed and analyzed by flow cytometry. b Percent specific lysis, P < 0.05 between (R)-DOTAP and (S)-DOTAP groups. No statistical difference exists between the two (R)-DOTAP-treated groups, or the (S)-DOTAP tumor-bearing group and tumor-bearing-untreated group. n = 15–18