Table 2.
Duplex and multiplex analyses of FFPE samples from patients with a tumor harboring an EGFR Exon 19 deletion.
| Sample# | Amount of input DNA (ng) | Tumor mutation (Real-time PCR) | Duplex analysis |
Multiplex analysis |
|---|---|---|---|---|
| Mutation (%) | Mutation (%) | |||
| 1 | 72.8 | Ex19del, T790M | Ex19del (30.334), T790M (5.284) | Ex19del (29.686), T790M (5.382) |
| 2 | 214.3 | Ex19del | Ex19del (19.068), T790M (1.375) | Ex19del (17.930), T790M (1.440) |
| 3 | 135.7 | Ex19del | Ex19del (19.746), T790M (0.307) | Ex19del (22.187), T790M (0.302) |
| 4 | 216.0 | Ex19del | Ex19del (28.217), T790M (0.135) | Ex19del (31.216), T790M (0.117) |
| 5 | 153.6 | Ex19del | Ex19del (3.674), T790M (0.073) | Ex19del (4.131), T790M (0.091) |
| 6 | 231.8 | Ex19del | Ex19del (37.183), T790M (0.024) | Ex19del (37.896), T790M (0.026) |
| 7 | 268.0 | Ex19del | Ex19del (40.174), T790M (0.041) | Ex19del (38.940), T790M (0.030) |
| 8 | 311.6 | Ex19del | Ex19del (11.818), T790M (0.046) | Ex19del (12.522), T790M (0.054) |
| 9 | 141.7 | Ex19del | Ex19del (37.136), T790M (0.041) | Ex19del (38.142), T790M (0.040) |
| 10 | 187.7 | Ex19del | Ex19del (25.069) | Ex19del (22.298) |
| 11 | 153.2 | Ex19del | Ex19del (37.218) | Ex19del (35.742) |
| 12 | 123.1 | Ex19del | Ex19del (32.418) | Ex19del (32.788) |
| 13 | 113.0 | Ex19del | Ex19del (38.710) | Ex19del (37.505) |
| 14 | 169.2 | Ex19del | Ex19del (16.112) | Ex19del (15.451) |
| 15 | 123.4 | Ex19del | Ex19del (44.617) | Ex19del (43.660) |
| 16 | 100.2 | Ex19del | Ex19del (16.992) | Ex19del (15.538) |
| 17 | 78.5 | Ex19del | Ex19del (28.056) | Ex19del (28.622) |