Table 2.

Increased SNO proteins in EDC-treated ovariectomized hearts by 2D CyDye-maleimide DIGE

Spots #	Protein Name	Protein ID	Score	Coverage	Unique peptides	#PSM	Mw (KDa)	Cal. pI	SNO level (EDC vs Vehicle)
1	Aconitase	Q99KI0	4001.3	36.4	26	185	85.4	7.93	2.6 ± 0.5
2	Trifunctional enzyme subunit α	Q8BMS1	17735.8	37.2	35	606	82.6	9.14	2.9 ± 0.6
3	Complex I-75 kDa subunit	Q91VD9	914.9	22.4	17	48	79.7	5.72	1.9 ± 0.4
4	F0F1 ATPase subunit α	Q03265	4805.4	34.0	23	197	59.7	9.19	2.7 ± 0.7
5	Trifunctional enzyme subunit β	Q99JY0	6171.2	42.5	33	269	51.4	9.38	3.0 ± 0.9
6	Isocitrate dehydrogenase [NADP], mitochondrial	P54071	5577.3	35.0	17	208	50.9	8.69	2.0 ± 0.4
7	Long-chain specific acyl-CoA dehydrogenase	P51174	12935.2	38.1	16	368	47.9	8.31	1.8 ± 0.3
8	Creatine kinase M- type	P07310	3746.1	38.3	18	170	43.0	7.06	2.5 ± 0.6
9	Actin, cytoplasmic 1	P60710	6797.4	28.8	13	252	41.7	5.48	2.7 ± 0.4
10	Malate dehydrogenase, cytoplasmic	P14152	1728.2	29.9	10	122	36.5	6.58	3.1 ± 0.9
11	Glyceraldehyde-3- phosphate dehydrogenase	P16858	4175.6	28.2	10	126	35.8	8.25	1.6 ± 0.2
12	Malate dehydrogenase, mitochondrial	P08249	2069.1	42.0	14	84	35.6	8.68	1.5 ± 0.1
13	Tropomyosin α-1 chain	P58771	6608.5	62.7	18	258	32.7	4.74	1.9 ± 0.3
14	Troponin I, cardiac muscle	P48787	7350.7	55.0	20	312	24.2	9.55	1.6 ± 0.3
15	Myosin regulatory light chain 2, cardiac isoform	P51667	3353.0	52.4	11	185	22.4	5.10	1.7 ± 0.3
16	Myoglobin	P04247	4315.6	57.1	13	122	17.1	7.62	1.7 ± 0.2

Note: Positive identifications from three independent experiments consisted of two peptides or more with the highest MS/MS score and a correct molecular mass position.