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. Author manuscript; available in PMC: 2017 Jul 18.
Published in final edited form as: Plant J. 2017 Apr;90(1):133–146. doi: 10.1111/tpj.13481

Figure 6. Arabidopsis TE-associated lincRNA11195 mediates ABA responses.

Figure 6

(a) Expression analysis of the TE-associated lincRNA11195 in wild-type (Col-0) and two T-DNA insertion mutant alleles (lincRNA11195-1 and 11195-2). Bold right curved arrow shows the direction of transcription of lincRNA11195. The two primer pairs shown (1 and 2 for 11195-1 and 3 and 4 for 11195-2) were used to amplify the TElincRNA11195 transcript. UBQ5 was used as a positive control.

(b) TE-linc11195 mutants are insensitive to ABA.

(c) Root length and fresh shoot weight of seedlings shown in (b). Both graphs are presented as the percentage relative to growth on control half-strength MS medium. ABA assays were performed by stratifying seeds at 4°C for 3 days, followed by growth of seedlings for 5 days on half MS media, then seedlings were transferred onto half-strength MS medium supplemented with or without 20 μM ABA, and grown for an additional 8 days. Error bars stand for standard deviation (n = 20). [Colour figure can be viewed at wileyonlinelibrary.com].