FIG 1.
NRF2 activation in representative tissues of Sf mice in the Keap1 knockdown background. (A) Gene expression of Keap1 and an NRF2 target gene, Nqo1, in WT (n = 4), Keap1FA/− (n = 4), Sf (n = 4), and Sf::Keap1FA/− (n = 4) mice. The expression levels were normalized to Gapdh expression, and the expression level in WT mice was set to 1. The values represent the means and SD. (B) Immunoblot analysis of the NRF2 protein in WT, Keap1FA/−, Sf, and Sf::Keap1FA/− mice. α-Tubulin was used as a loading control. *, P < 0.05; **, P < 0.005; ***, P < 0.0005.