FIG 10.

Mutant Tfe3 (MAPK phospho acceptor serine to alanine) does not transcriptionally stimulate the Pparγ2 regulatory region. (A) The murine Pparγ2 regulatory region (intron 1) construct was used (see Fig. 9). 3T3-L1 preadipocytes were cotransfected with the SV40 promoter/luciferase vector containing the Pparγ2 regulatory region, the control Renilla, and the Tfe3 and/or mutant Tfe3 expression vectors or empty expression vector (control). Luciferase activity was normalized to Renilla activity to control for transfection efficiency. The level of luciferase activity of the empty vector control was defined as “1.” Fold activation was estimated to this level of activity. Values are means ± SD (n = 3). (B) Western blot showing equal levels of Tfe3 (wild type) and Tfe3 (serine-to-alanine mutant).