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. 2017 Jul 17;22:12. doi: 10.1186/s11658-017-0041-5

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — SDAD1 promotes the proliferation, migration and invasion of colon cancer cells. a The expression of SDAD1 in 27 pairs of colon cancer and adjacent non-tumor tissues was determined using quantitative RT-PCR. b The MTT assay was performed on SW480 and HCT-116 cells transfected with SDAD1 overexpression plasmid. c A colony formation assay was performed on SDAD1 overexpression plasmid-transfected SW480 and HCT-116 cells. d FACS was performed to analyze the apoptosis of SW480 cells when SDAD1 was overexpressed. e The effect of SDAD1 transfection on the migration/invasion of SW480 and HCT-116 cells was determined using Transwell plates. f Western blot was used to measure the protein levels of the EMT protein makers in SW480 cells transfected with SDAD1 overexpression plasmid. *p < 0.05, **p < 0.01, ****p < 0.0001