Fig. 4.

miR-367 was a target of CASC2 in HCC. a Bioinformatics analysis showed that miR-367 could directly target 3′-UTR of CASC2-wild type (WT). CASC2-mutant (Mut) means mutation of binding sites in the 3′-UTR of CASC2. b The expression of miR-367 in tumor tissues was significantly higher than that in adjacent nontumor tissues. c Pearson correlation analysis revealed that an obvious negative association between miR-367 and CASC2 expression in HCC tissues. d The expression of miR-367 in LO2, MHCC-97H and Hep-3B cells as detected by real time-PCR. e miR-367 mimics or inhibitors could significantly modulate the expression of miR-367 in HCC cells. f Dual luciferase reporter assays showed that miR-367 could negatively regulate the luciferase activity of CASC2-WT, rather than CASC2-Mut. g Real-time PCR showed that CASC2 could negatively regulate miR-367 expression in HCC cells. h miR-367 inversely regulate CASC2 expression in HCC cells. *P < 0.05, **P < 0.01, ***P < 0.001