Fig. 6.

FBXW7 was a target of miR-367 and positively regulated by CASC2 in HCC cells. a Bioinformatics analysis showed that CASC2 could directly target 3′-UTR of FBXW7-wild type (WT). FBXW7-mutant (Mut) means mutation of binding sites in the 3′-UTR of FBXW7. b and c miR-367 negatively regulated the luciferase activity of FBXW7-WT, rather than FBXW7-Mut in HCC cells. d and e miR-367 negatively regulated the level of FBXW7 mRNA in HCC cells. f and g) miR-367 inversely regulated the expression of FBXW7 protein in HCC cells. h and i CASC2 positively regulated the level of FBXW7 mRNA in HCC cells. j and k CASC2 positively regulate FBXW7 abundance in both MHCC-97H and Hep-3B cells. **P < 0.01, ***P < 0.001