Table 1.
Bisulfite sequencing PCR primers and PCR conditions for BAS samples
| Gene | Sequences (5′ to 3′) | Product size (bp) | Annealing (°C) | Annealing Time (s) | Position from TSS | UCSC gene ID |
|---|---|---|---|---|---|---|
| CR2 | F: GGGTGAGTTTGAGTTAAAGAGTGG R: AAAAAACCAATAAAAACAATCAAAACCAAA |
514 | 58 | 50 | −149 – +365 | uc001hfv.3 |
| ITGA4 | F: TGTAATTTTGGGGTAGTGGT R: CCCTCCTACCTCCTTAAAAAAAAAAAA |
362 | 58 | 45 | +711 − +1072 | uc002unu.3 |
| RERG | F: GGAGTTTGGAGGTTTGGAAAT R: CAAAAACAAATACCAATAACCC |
278 | 58 | 45 | −145 – +133 | uc001rct.3 |
| RRAD | F: TTGGTGGGGGTGGATAGATA R-CCTCCCCCAACCCCCAAAT |
331 | 61 | 45 | −102 – +228 | uc002eqo.2 |
| SHISA3 | F: GGTTGAGAGTTAAGTTTTGGGGG R: CCTCCCCACTCCTCAAAAAAA |
446 | 58 | 45 | −570 – −125 | uc003gwp.3 |
| ZNF549 | F: TTTTAGTTTGATGGGTTTTTTTTTTTGTT R: AAACCTCAAAACCCAAATAAAAATC |
502 | 56 | 45 | −177 – +325 | uc002qpb.2 |
| ZNF671 | F: ATTTTGTTTTTGTTAGGTTGTTTTTGG R: CTATCCTAAAACACAAAAACTACAAACACT |
311 | 57 | 45 | +13 − +323 | uc002qpz.4 |
PCR cycles: 40
CR2: complement C3d receptor 2, ITGA4: integrin subunit alpha 4, RERG: RAS-like estrogen regulated growth inhibitor, RRAD: Ras-related glycolysis inhibitor and calcium channel regulator, SHISA3: shisa family member 3, ZNF549: zinc finger protein 549, ZNF671: zinc finger protein 671