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. 2017 Jul 17;15:52. doi: 10.1186/s12958-017-0273-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — FACS sorting of CD13+ and CD146+/PDGFR-β + cells from mouse EBs. Total EB cells gated as DAPI+/CD45−/CD13+ (a) and DAPI+/CD45−/CD146+/PDGFR-β + (b) are shown. Graph legends indicate percent of parent population. c shows the proportion of CD13+ and CD146+/PDGFR-β + cells isolated from both the total cell population and viable CD45- population in EB medium by flow cytometry. Quantitative RT-PCR revealed significantly up-regulated expression of Hoxa10, Foxa2, ER-A, PR-A and PR-B, in a CD13+ (stromal) subpopulation (d). The x-axis represents the gene and the y-axis represents the relative fold change to the Beta-actin gene control. Standard deviations of means are shown with brackets