Table 1.

Peptides and transition ions used for quantitative MRM analysis

Peptide	Phosphosite(s)	Parent m/z	Transition	Transition m/z	Collision Energy
VLTVPC[+57]QK	Control	472.765	b2	213.16	14
VLTVPC[+57]QK	Control	472.765	y4	532.255	17
VLTVPC[+57]QK	Control	472.765	y6	732.371	14
IGAQM[+16]GHPGS[+80]PNAHPVTWSPGGIISR	S270	907.43	y12	1269.695	37
IGAQM[+16]GHPGS[+80]PNAHPVTWSPGGIISR	S270 or T277 or S279	907.43	y7	699.415	38
IGAQM[+16]GHPGS[+80]PNAHPVTWSPGGIISR	S270 or T277 or S279 or S285	907.43	b7	711.324	36
IGAQM[+16]GHPGSPNAHPVTWS[+80]PGGIISR	T288 or S279 or S285	907.43	y10-98	1055.563	41
IGAQM[+16]GHPGSPNAHPVTWS[+80]PGGIISR	T277 or S279 or S285	907.43	y12-98	1251.684	37
EALPM[+16]DTEVY[+80]ESPYADPEEIRPK	T339 or Y342 or S344 or Y346	925.742	b2	201.087	38
EALPM[+16]DTEVY[+80]ESPYADPEEIRPK	T339 or Y342 or S344 or Y346	925.742	y4	513.351	38
EALPM[+16]DTEVY[+80]ESPYADPEEIRPK	T339 or Y342 or S344 or Y346	925.742	y7	868.489	42
EALPM[+16]DTEVY[+80]ESPYADPEEIRPK	T339 or Y342 or S344 or Y346	925.742	y20	1231.527	38
EALPM[+16]DTEVYESPY[+80]ADPEEIRPK	S344 or Y346	925.742	y12	741.337	38
EALPM[+16]DTEVYESPY[+80]ADPEEIRPK	Y346	925.742	y10	649.295	39
EALPM[+16]DTEVYESPY[+80]ADPEEIRPK	Y346	925.742	y11	697.821	38
ALRADENY[+80]YK	Y519	661.792	y2	310.176	28
ALRADENY[+80]YK	Y519 or Y520	661.792	b5	527.294	23
ALRADENY[+80]YK	Y519 or Y520	661.792	b6	656.336	23
ALRADENYY[+80]K	Y519 or Y520	661.792	y3	277.107	24
ALRADENYY[+80]K	Y519 or Y520	661.792	y3	553.206	20
ALRADENYY[+80.0]K	Y520	661.792	y2	390.142	26
ALRADENY[+80.0]Y[+80.0]K	Y519 + Y520	701.776	b3	341.23	24
ALRADENY[+80.0]Y[+80.0]K	Y519 + Y520	701.776	b5	527.294	20
ALRADENY[+80.0]Y[+80.0]K	Y519 + Y520	701.776	b6	656.336	20
LRNYY[+80.0]YDVVN	Y623 or Y624 or Y625	699.808	b7	1068.419	24
LRNYY[+80.0]YDVVN	Y623 or Y624 or Y625	699.808	b8	1167.487	21
LRNYY[+80.0]YDVVN	Y623 or Y624 or Y625	699.808	y2	232.129	24