Table 3.
Cytotoxic activity of crude extract of Z. armatum using (NRU) neutral red uptake assay with apparent IC50 100 μg/ml
| Conc. (μg/ml) | Z. armatum fruit saponins (Zf.Sa) | Z. armatum leaves saponins (Zl.Sa) | Z. armatum bark saponins (Zb.Sa) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| MCF-7 cells | MDA MB-468 cells | Caco-2 cells | MCF-7 cells | MDA MB-468 cells | Caco-2 cells | MCF-7 cells | MDA MB-468 cells | Caco-2 cells | |
| 10 | 16.02* ± 7.5 | 13.98 ± 3.9 | 25.76* ±2.1 | 2.71 ± 0.5 | 13.92 ± 3.6 | 5.74 ± 4.0 | 20.04* ±4.4 | 4.43 ± 1.4 | 3.64 ± 0.4 |
| 25 | 27.89* ±6.1 | 29.60* ±3.9 | 26.60* ± 4.2 | 3.07 ± 1.2 | 28.27* ± 3.2 | 7.42 ± 1.7 | 45.78** ±5.1 | 6.31 ± 1.2 | 4.46 ± 3.0 |
| 50 | 46.82** ±6.1 | 39.31** ± 3.9 | 30.52** ± 4.3 | 3.51 ± 1.4 | 25.76* ± 4.1 | 13.87 ± 5.0 | 66.05*** ±6.0 | 9.55 ± 0.9 | 4.03 ± 3.8 |
| 100 | 58.08*** ±7.1 | 53.53*** ±3.9 | 42.99** ± 3.3 | 4.27 ± 2.1 | 52.61** ± 5.6 | 10.18 ± 4.1 | 72.23*** ±3.2 | 28.91* ±5.0 | 19.59 ± 3.1 |
| 200 | 58.75*** ±4.0 | 55.75*** ±3.9 | 59.78*** ±6.7 | 34.39** ±2.9 | 77.43*** ± 5.4 | 32.90** ± 8.2 | 83.29*** ±3.6 | 99.36*** ±0.6 | 22.85 ± 5.1 |
| 300 | 71.62*** ±4.8 | 66.62*** ± 3.9 | 72.47*** ±5.6 | 93.73*** ±2.0 | 99.04*** ± 1.6 | 30.13** ± 2.5 | 92.55*** ±5.8 | 100.00*** ±0.2 | 30.98** ±8.4 |
| 400 | 80.89*** ±6.2 | 73.72*** ±3.9 | 91.78*** ±3.6 | 100.71*** ± .01 | 100.14*** ± 1.3 | 56.45*** ± 2.6 | 98.78*** ±1.2 | 100.00*** ±0.3 | 66.03*** ±5.4 |
| 500 | 86.18*** ±4.6 | 93.29*** ±3.9 | 96.86*** ±2.9 | 100.81*** ±0.2 | 100.14*** ± 0.5 | 63.97*** ± 3.4 | 99.08*** ±1.0 | 100.00*** ±0.6 | 90.01*** ±3.1 |
| Veh. Cont. | −1.49 ± 1.0 | 1.38 ± 0.9 | −3.45 ± 1.8 | 4.41 ± 2.2 | 1.91 ± 1.6 | 1.35 ± 3.7 | 1.72 ± 1.4 | 5.21 ± 3.0 | −1.81 ± 0.9 |
In the table statistically (Dunnett’s multiple comparison test) * = Significant (P < 0.05), ** = Highly significant (P < 0.01), *** = Very highly significant (P < 0.001)