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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Nat Protoc. 2017 Apr 6;12(5):916–946. doi: 10.1038/nprot.2017.017

Figure 1.

Figure 1

Flowchart of the protocols featured in this paper. See text and Table 1 for the details, advantages and limitations, and anticipated results for each procedure. Scale bars = 500 nm. FIB-SEM/iPALM image in bottom panel is reproduced from19. Arrows in TEM/PALM image indicate the locations of mitochondrial cristae. Images further discussed in Figure 6. Au, gold; C, carbon; HPF/FS, high-pressure freezing/freeze substitution; LN2, liquid nitrogen; OsO4, osmium tetroxide; Pb, lead; Pd, palladium; Pt, platinum; UA, uranyl acetate.