Figure 2.
Example of using gold nanoparticles to align fluorescence and electron microscopy datasets. (a) Scanning electron micrograph where gold nanoparticles can be observed as white dots (arrows) due to their high electron densities. (b) Fluorescence signal from gold nanoparticles (arrows) with a DIC image showing cell outlines. Note this image shows diffraction-limited fluorescence for illustration purposes, as a super-resolution fluorescence rendering would make the gold nanoparticles difficult to see. Scale bars = 5 μm. Figure reproduced with permission from18.