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Interaction Analysis of DMBT1 Peptides with tdTomato1 siRNA
(A and B) Electrophoretic mobility shift assay of SRCRP2-11 (A) and SRCRP2-11-R (B) incubating tdTomato1 siRNA (5 μM) with increasing amounts of peptide. (C and D) UV spectra analysis shows hypochromicity of the siRNA peak at 260 nm. (E and F) Hypochromicity can be highlighted by plotting the relative change in absorbance, ΔODr, against the peptide/siRNA molar ratio.
