Figure 3.

Evaluation of DMBT1-Peptide-tdTomato1 siRNA Complex Stability in the Presence of RNase A or FBS

(A and B) SRCRP2-11-siRNA (A) and SRCRP2-11-R-siRNA (B) complexes were formed at peptide-siRNA molar ratios between 200 and 800, corresponding to increasing amounts of peptide. The complexes were incubated 2 hr at 37°C in the presence of RNase A in concentrations corresponding to serum levels ranging from normal subjects to patients with cancer (100–800). Stability was evaluated as the lack of free siRNA and the strength of the shifted band signal. (C and D) The experiment was repeated analogously in the presence of 10%–50% FBS.