Figure 4.

Dynamic Light Scattering, ζ-Potential, and Circular Dichroism Characterization of DMBT1 Peptide-tdTomato1 siRNA Complexes and the Proposed 3D Model

(A and B) Dynamic light scattering for SRCRP2-11-siRNA (A) and SRCRP2-11-R-siRNA (B) complexes showed dependence of the average diameter on the molar ratio and on the siRNA concentration. (C and D) ζ-potential was also sensitive to these two parameters. (E and F) Circular dichroism showed that conformation of both peptides changed from a random coil to a β-helix when the siRNA was gradually added. (G and H) Based on the conformational analysis, we built a 3D model for SRCRP2-11-siRNA (G) and SRCRP2-11-R-siRNA (H), which assumed a central siRNA surrounded by peptide molecules disposed parallel to each other. d.nm., diameter in nanometers.