TABLE 4.
Primer sets used for SCCmec typing of isolates
| Primer name | Nucleotide sequence | Expected product (bp) (forward primer used) | Source |
|---|---|---|---|
| ccrB-F | ATTGCCTTGATAATAGCCITCT | Ito et al. (37) | |
| ccrA1-R | AACCTATATCATCAATCAGTACGT | 694 (ccrB-F) | Ito et al. (37) |
| ccrA2-R | TAAAGGCATCAATGCACAAACACT | 937 (ccrB-F) | Ito et al. (37) |
| ccrA3-R | AGCTCAAAAGCAAGCAATAGAAT | 1,791 (ccrB-F) | Ito et al. (37) |
| ccrA4-R | GTATCAATGCACCAGAACTT | 1,287 (ccrB-F) | Kondo et al. (38) |
| ccrC-F | CGTCTATTACAAGATGTTAAGGATAAT | Kondo et al. (38) | |
| ccrC-R | CCTTTATAGACTGGATTATTCAAAATAT | 518 (ccrC-F) | Kondo et al. (38) |
| mecI-F | CAAGTGAATTGAAACCGCCT | Okuma et al. (39) | |
| mecI-R | CAAAAGGACTGGACTGGAGTCCAAA | 187 (mecI-F) | Okuma et al. (39) |
| mecR1-R | GTCTCCACGTTAATTCCATT | 1,920 (mecI-F) | Kobayashi et al. (40) |
| Class B-F | TATACCAAACCCGACAAC | Katayama et al. (41) | |
| IS1272-R | AACGCCACTCATAACATATGGAA | 1,996 (class B-F) | Okuma et al. (39) |
| Class C-F | AACGTTGTAACCACCCCAAGA | Hiramatsu et al. (42) | |
| IS431-R | TGAGGTTATTCAGATATTTCGATGT | 2,072 (class C-F) | Katayama et al. (41) |