FIG 3.

MidA and CchA are required for the extracellular calcium-based restoration of the DTT/thermal stress sensitivity in the ΔclxA mutant. (A) Colony morphology comparison for the indicated strains grown on solid MM in the presence or absence of 200 mM CaCl₂ at 30 or 42°C for 2.5 days. (B) Quantitative total conidial production for the strains shown in panel A. (C) Colony morphology comparison for the indicated strains grown on solid MM in the presence or absence of 200 mM CaCl₂ and/or 2 mM DTT at 30°C for 2.5 days. (D) Quantitative total conidial production for the strains shown in panel C. Error bars represent standard deviation from three replicates. Different lowercase letters on the bars of each group represent significant differences among strains (Tukey's multiple-comparison test, P < 0.05).