FIG 3.

Expression levels of ChiA, Dac, GlmA, and GlmD in engineered T. kodakarensis strains. All strains were grown in ASW-VMT-Mdx-CO medium. Western blot analysis of ChiA was performed using culture supernatants (CS). Culture supernatant corresponding to a volume of 150 μl was loaded on each lane. Western blot analyses of Dac, GlmA, and GlmD were performed using cell extracts (CE). An equal amount (10 μg) of protein was loaded on each lane. A plus sign indicates overexpression of the enzyme performed by promoter replacement, and a minus sign indicates that there is no promoter replacement. Numbers in parentheses indicate band intensities (percent) relative to those of KU216 cultivated under the same medium condition (which is defined as 100%).