FIG 6.

Quantification of acetate and ammonium concentrations in culture supernatants of engineered strains of T. kodakarensis growing on swollen chitin. Cultivations were performed in ASW-VMT-SC medium at 85 °C for 72 h, and culture supernatants prepared were analyzed. Results shown were determined by subtracting acetate or ammonium concentrations present in culture medium without inoculation. Error bars represent standard deviations of three independent measurements. KC04ΔtM1 showed increased production of both acetate and ammonium.