TABLE 3.
Primers used in the study
| Name | Sequence (5′–3′)a |
|---|---|
| ChiA_UP1_PstI | AAACTGCAGCCTTTCCTTTCTTATCACGG |
| PcsgR | GTGGTGGTGGTGGTGGTGCATATGACAACACCTCCTTGGGTT |
| OE-chiA-f-1 | AAAAGTCGACCATATGAAGAAGATTTGGACTTCA |
| OE-chiA-r-1 | AAAAGAATTCAGAGGATCAGCGTAGGTATC |
| dPchiA-inverse-f | AAAAAGATCTTATCGGCAAAAGGCGAATTATGTGT |
| dPchiA-inverse-r | ATGGTGTTTGAGGAGTTCAACAATT |
| PEF-1a-EcoRV-f | AAAAGATATCCAAACACCTCCATATTTTGGT |
| PEF-1a-r | AAAAAGATCTTGCGGGCTTTCTTCTTGTTCTCTC |
| ABCtransporter-f | AAAACTGCAGTATGTCGATGTAGTATGTACCGACG |
| ABCtransporter-r | AAAAGAATTCTGAGAAGGTTTTAACTGCCTCGTAG |
| ABCt-inverse-f | AAAAGGATCCCATATGAAGAAAGCTACCGCGGTTG |
| ABCt-inverse-r | AAAAGGATCCGGACACCACCACTTATAAGG |
| Pgdh-f | AAAAGTCGACCATATGTCATACCACCTCATTTCGGTAAT |
| Pgdh-r | AAAAGGATCCGCCCGTTGCCCGATGATTGGTTT |
| Δtk1769.1 | AAAAAAGTCGACGGTGGAAAACGCCGTCGAGTAC |
| Δtk1769.2 | TTTTTTGGATCCCGGTTATCACTTTCACGTTCTC |
| Δtk1769.3 | CCCATCATTTTTAATTTCTAAACTT |
| Δtk1769.4 | GGCTTAACCCCCAAAGACATTTAAG |
| TK1769compF1 | GGGAATTCGCGGCCGCCACTCTGCCGTGGATGAAGT |
| TK1769compR1 | GCATGGGCCCTAAAGCTATGCCCCAAAAATTCAGAAGAGAAATAGAAAAATGTAGAGGAATCACTCAAGGAGGATGAACT |
a
Restriction enzyme sites incorporated for genetic manipulation are underlined.