TABLE 1.

Growth of Methanococcus maripaludis strain S2 in 96-well microtiter plate format

Replicate	Final OD600a
	Medium only	Inoculum only (4%)	DMSO (2%)	Monensin (1 μM)	BES (30 μM)
R1	0	0.702	0.553	0.015	0.039
R2	−0.003	0.661	0.590	0.025	0.054
R3	−0.002	0.555	0.550	0.025	0.058
R4	−0.003	0.589	0.415	0.018	0.053
R5	−0.003	0.455	0.417	0.034	0.045
R6	−0.004	0.521	0.442	0.025	0.050
R7	−0.002	0.503	0.417	0.015	0.062
R8	−0.002	0.528	0.602	0.012	0.044
Avg	−0.002	0.564	0.498	0.021	0.051
SD	0.001	0.083	0.083	0.007	0.008

^aRecorded after 5 days of incubation at 37°C using a 4% inoculum (approximate OD₆₀₀ of 0.020) and McF medium containing 0.2% sodium sulfide (wt/vol) and 400 mM sodium formate. The Z′ for the assay was 0.64.