TABLE 2.

Growth of Methanobrevibacter species AbM4 in 96-well microtiter plate format

Replicate	Final OD600a
	Medium only	Inoculum only (2.5%)	Inoculum only (5%)	Inoculum only (10%)	DMSO (2%)	Monensin (1 μM)	BES (30 μM)
R1	0	0.179	0.376	0.451	0.254	0.039	0.062
R2	−0.003	0.161	0.370	0.442	0.278	0.045	0.049
R3	−0.002	0.174	0.379	0.438	0.282	0.040	0.061
R4	−0.003	0.275	0.397	0.348	0.285	0.038	0.053
R5	−0.003	0.157	0.421	0.479	0.292	0.038	0.062
R6	−0.004	0.149	0.430	0.408	0.280	0.041	0.069
R7	−0.002	0.157	0.365	0.411	0.257	0.016	0.053
R8	−0.002	0.190	0.428	0.398	0.254	0.026	0.049
Avg	−0.002	0.180	0.396	0.422	0.273	0.035	0.057
SD	0.001	0.041	0.027	0.040	0.015	0.010	0.007

^aRecorded after 4 days of incubation at 38°C using a 5% inoculum (approximate starting OD₆₀₀ of 0.020) in medium containing reductant (0.05% cysteine [wt/vol]). The Z′ was 0.82.