FIG 3.

Quantification of polysaccharides. (A) Comparison of the soluble and insoluble polysaccharides synthesized in TSBs, TSBg, and TSBr. TSBs and TSBg serve as a positive control and negative control for the synthesis of polysaccharides, respectively. (B) Discrimination between glucan and fructan among the soluble polysaccharide synthesized in TSBr. We performed quantification of the soluble polysaccharide synthesized in TSBr using the ΔgtfD mutant, which cannot synthesize a soluble glucan, and the ΔsacB mutant, which cannot synthesize fructan. These data are presented as the means ± SD of the results from three independent experiments. The asterisks indicate a significant difference between two groups (Student's t test, P < 0.05).