TABLE 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| P. chlororaphis 30-84 | Wild type; Rifr | W. W. Bockus |
| P. chlororaphis 30-84 ΔHolin | hol gene replaced with Kmr cassette; Kmr | 29 |
| P. chlororaphis 30-84 ΔTF1 | TF1 gene replaced with Kmr cassette | This study |
| P. chlororaphis 30-84 ΔTF2 | TF2 gene replaced with Tcr cassette | This study |
| P. chlororaphis 30-84 ΔTF1/2 | TF1 and TF2 genes replaced with Kmr and Tcr, respectively | This study |
| P. chlororaphis 30-84 ΔBP1 | BP1 genes replaced with Kmr cassette; Kmr | This study |
| P. chlororaphis 30-84 ΔBP2 | BP2 genes replaced with Tcr cassette; Tcr | This study |
| P. chlororaphis 30-84 ΔBP1/2 | BP1 and BP2 genes replaced with Kmr and Tcr, respectively | This study |
| Escherichia coli DH5α | F− recA1 endA1 hsdRl7 supE44 thi-J gyrA96 reLA1A (argF-lacZYA) 1169 + 801acZAM15/− | GIBCO-BRL |
| Escherichia coli HB101 | F− hsdS20(rB−mB−) supE44 recAl aral4proA2 lacYl galK2 rpsL20 xyl-5mtl-5I−; GIBCO-BRL | GIBCO-BRL |
| P. putida F1 | Rhizosphere associated | ATCC |
| P. putida KT2440 | Rhizosphere associated | D. C. Gross (Plant Pathology and Microbiology, TAMU) |
| P. syringae pv. tomato | Plant pathogen | 43 |
| P. syringae pv. phaseolicola N4SP | Plant pathogen | Teaching collectionsa |
| P. marginalis L | Plant pathogen | Teaching collections |
| P. syringae pv. syringae B728A | Plant pathogen | 44 |
| P. syringae pv. tomato DC3000 | Plant pathogen | 45 |
| P. aeruginosa PAK | Opportunistic human pathogen | 46 |
| P. aureofaciens ATCC 13485 | Rhizosphere associated | ATCC |
| P. fluorescens BL915 | Rhizosphere associated | 47 |
| P. syringae pv. tabaci | Plant pathogen | Teaching collections |
| P. protegens Pf-5 | Rhizosphere associated | 24 |
| P. fluorescens 2-79 | Rhizosphere associated | 25 |
| P. aeruginosa PAO1 | Opportunistic human pathogen | 48 |
| P. fluorescens Q287 | Rhizosphere associated | 49 |
| P. fluorescens F113 | Rhizosphere associated | 50 |
| P. tolaasii NCPPB 1116 | Fungal pathogen | 51 |
| P. aureofaciens Z1B | Rhizosphere associated | Pierson Lab |
| Bacillus megaterium | Rhizosphere associated | Teaching collections |
| Bacillus subtilis 613R | Rhizosphere associated | Teaching collections |
| Pectobacterium carotovorum ATCC 15713 | Plant pathogen | ATCC |
| Agrobacterium tumefaciens C58 | Plant pathogen | Teaching collections |
| Erwinia amylovora ea1189 | Plant pathogen | 52 |
| Plasmids | ||
| pEX18Ap | Apr | 38 |
| pUC4K | Kmr Apr aph | 53 |
| pKRP12 | Tcr | 54 |
| pUCP20Gm | Gmr | 39 |
| pHERD20TKm | pHERD20T derivative bearing Kmr cartridge; Kmr | This study |
| pHERD20TKmHE | pHERD20TKm containing 946-bp hol::endo fragment; Kmr | This study |
| pHERD20TKmE | pHERD20TKm containing 581-bp endo fragment; Kmr | This study |
| pTF1 | pUCP20Gm containing 3,092-bp tail fiber 1 and chaperone fragment; Gmr | This study |
| pTF2 | pUCP20Gm containing 4,141-bp tail fiber 2 and chaperone fragment; Gmr | This study |
a
University of Arizona School of Plant Sciences or Texas A&M University Department of Plant Pathology and Microbiology teaching collection.