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. 2017 Jul 18;7:323. doi: 10.3389/fcimb.2017.00323

Figure 2.

Figure 2

Orally fed LGG reduces the number of Salmonella in the jejunal mucosa but increases the fecal abundance of Bifidobacterium during S. Infantis infection. Fresh fecal samples from animals of the indicated groups (n = 6 per group) were collected on days 1, 4, 8 (0, 6, 12, and 24 h after infection), 13, and 16 after weaning. The mucosal tissues of the midjejunum and distal ileum were collected from the indicated pigs 10 days after S. Infantis challenge. The numbers of Salmonella (A,C) and Lactobacilli (B,D) in intestinal mucosal tissues or fecal shedding after S. Infantis challenge were monitored using culture-based enumeration. Bacterial DNA isolated from 200 mg of feces from pigs of the four groups was analyzed by quantitative PCR using universal primers for bacterial 16S rRNA genes (E–G). Data are presented as means ± SEM. *P < 0.05; Mean values at the same time point without a common superscript letter differ significantly (Tukey's test).