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. 2017 Jun 23;114(28):E5673–E5682. doi: 10.1073/pnas.1607002114

Fig. 7.

Fig. 7.

Reproductive experience enhances revascularization after stroke. Poststroke angiogenesis was assessed at 30 d in animals treated with BrdU and i.v. FITC-lectin injection. Representative microphotographs show FITC-labeled vessels in the ischemic cortex of each group (A). (Magnification: 20×.) AngioTool software was used to make quantitative measurements and perform analyses as illustrated (in red). The percent area containing vessels was quantified (B). The total number of junctions (C) and endpoints (D) is shown. Total vessel length was increased in multiparous females after stroke (E). Representative images of the ischemic penumbra (63× magnification) reveal colabeling of FITC-lectin-stained (green) functional blood vessels with BrdU+ (red) and DAPI+ (blue) nuclei as further highlighted by the white arrows (F). (Scale bar, 10 μm.) Quantification of colabeled BrdU+ cells is shown (G, n = 5 per group). Error bars show mean SEM. *P < 0.05; **P < 0.01.