Fig. S1.

No overt difference in microglia/macrophage counts between virgin and multiparous brains. Representative dot plot shows the relative density of CD45^intCD11b⁺ microglia, CD45^hiCD11b⁺ myeloid cells, and CD45^hiCD11b⁻ lymphocytes in the normal brain of virgin and multiparous females (A). The absolute number of perivascular macrophages (CD45^hiCD11b⁺Ly6C⁺), lymphocytes (CD45^hiCD11b⁻), and microglia (CD45^intCD11b⁺Ly6C⁻) per hemisphere were quantified (B–D, respectively). To assess physical properties of microglia activation, the relative cell size [forward light scatter intensity (FSC)] and granularity [side light scatter intensity; (SSC)] was evaluated (E and F, respectively). For all experiments, n = 5 per group. Error bars show mean SEM. a.u.i., arbitrary unit of intensity; n.s., not significant.