Table S2.

Primer sequences

Primer	Sequence, 5′-3′
Transcription level comparison between human (293T) and pig (PIEC) cell lines
Ku70
70-QSF	AGTGACAGCTTTGAGAACCC
70-QSB	GGATTGTAATCTGGTGGGTA
Ku80
80-QSF	TGGCTCCAATTTGTCTATAAGG
80-QSB	CCATAGCGGAACCCTTGAATA
DNA-PKcs
PK-QSF	GTTAGCAGGTGCCAATCCAGC
PK-QSB	GTTGGGGTCTGTTGCCTGGTC
Actb
Act-QSF	AAGGAGAAGCTGTGCTACGTCG
Act-QSB	TGAAGGTAGTTTCGTGGATGCC
Transcription level comparison between human (293T) and chicken (DF-1) cell lines
Ku70
70-2QF	TAGCAGAGGATGAGGACCT
70-2QB	CGCTAGGCAGAAGCAAAC
Ku80
80-2QF	TCCAATTTGTCTATAAGGATTG
80-2QSB	AAAGGAACTATATCACT
DNA-PKcs
PK-2QF	GAGAATCAGCTGCTGGAGA
PK-2QB	TGACTCATTTCCTCACTCA
Actb
Act-2QF	GACAGGATGCAGAAGGAGAT
Act-2QB	TACTCCTGCTTGCTGATCCA
Mouse genotyping
Ku80
Wild-type band
216-F3	GCCAGCCTCCCCCTAATACAGATGAGAGAATATG
216-B10	TGCCTCCAGCTCTAACGGAAAG
Insertion band
Bac-RF1	CCTCGATATACAGACCGATAAAACACATGC
216-B3	TCAGTCTCTTTCAGGGTTACATGGTACTTCAGGGC
222 site
Wild-type band
222-L2A	GCCCTTGTTAAATACAGACAGATTC
222-R2A	GCACATAAACACGAGTATATCAGTG
Insertion band
222-L2A	GCCCTTGTTAAATACAGACAGATTC
Bac-RF1	CCTCGATATACAGACCGATAAAACACATGC
A6 site
Wild-type band
A6-F2	ACTGGTTACTTCATATTGATGTTCCTCCAC
A6-B2	CCCTCGATCTCTTTTTGTTGTCTTATTGTTCTAGC
Insertion band
A6-F2	ACTGGTTACTTCATATTGATGTTCCTCCAC
LB2	CTGAGATGTCCTAAATGCACAGCG
Testing DNA-PKcs RNAi vectors of HEK293T cells
DNA-PKcs
PK-E73	ACGGTATCCAGAGGAGACTTTGAGC
PK-E74	GCTTTCGCTGCTTATGATGAAGGG
GAPDH
GAPDH-E7	TGACAACTTTGGTATCGTGGAAGG
GAPDH-E8	GCAGGGATGATGTTCTGGAGAG
Testing mouse germ-line Ku80 transcription levels
Ku80
216-E6F	CACCTCAAACCCTCCTTTCCTC
216-E7B	AGACGCACAGTTGTCGTAGACTCTC
GAPDH
GAPDHQ-L1	TGTTCCTACCCCCAATGTGTCC
GAPDHQ-R1	GGAGTTGCTGTTGAAGTCGCAG