Fig. 3.
Regulation of GTPase activity of hGBP1F by polymerization and membrane interaction. (A) Absorbance signal of 10 μM hGBP1F after injection of nucleotides at t = 0 s. (B) Absorbance signal of 10 μM hGBP1F after injection of 1 mM GTP (t = 0 s) superimposed with nucleotide composition in the same solution. The plot includes data from three independent experiments; absorbance represents the average of all three datasets. (C) Absorbance signal of 10 μM hGBP1F after injection of 1 mM GTP in the presence of different concentrations of LUVs. The baseline of LUVs alone was subtracted from the raw data (Fig. S6 A and B). (D) GTP turnover from experiments described in C. (E) Enzymatic activity of 10 µM hGBP1F in the presence of different LUV concentrations. Specific activities of GTP hydrolysis in the slow and fast phases were calculated from the data presented in D.