Fig. 5.
Fast recovery of mCherry-hGBP1 on GFP-Rab7+ structures. (A) FRAP: time lapse of mCherry-hGBP1 and GFP-Rab7 fluorescence recovery on a vesicle in cotransfected HeLa cells after bleaching. The first picture of each panel shows the region of interest (dashed circle) before bleaching. Asterisks mark first detection of recovering signals. (Scale bars, 10 μm.) (B and C) Data plots showing the half-lives of fluorescence recovery (τ1/2) of cotransfected mCherry-hGBP1 and GFP-Rab7 on vesicular structures (B) and BCP signals (C) after photobleaching. Values obtained for both protein constructs in the same measurement are connected by lines. Orange squares indicate the mean values.