Fig. S4.
EM images of hGBP1. (A) Representative images of either hGBP1F or hGBP1NF incubated with the indicated nucleotides. Experimental mixture contained 10 μM of hGBP1F or hGBP1NF and 1 mM of corresponding nucleotide, except for samples with GDP·AlFx, which contained 200 μM GDP, 300 μM AlCl3, and 10 mM NaF. Reactions were stopped after ∼10 min (GTP) and after ∼20 min (GTPγS and GDP·AlFx). (Scale bars, 200 nm.) (B) Measures for the identified polymer structures (Fig. 2) of hGBP1F in the presence of indicated nucleotides as schematically drawn. The numbers of quantified structures (N in parentheses) as well as obtained mean values with SDs are indicated along each scheme.