Fig. S9.

Recruitment of endogenous hGBP1 to phagocytosed latex beads. HeLa cells were IFN-γ induced for 24 h and then incubated for 4 h with nonfluorescent (A and D) or blue-fluorescent (B, C, and E) latex beads with 2-µm (A–D) or 1-µm (E) diameter. The monoclonal 1B1-antibody against hGBP1 and several antibodies against distinct structures of the phagolysosomal pathway were used for costaining. (A) Rab9 (LE/TGN) and Rab5 (EE). (B) LAMP1 (lysosomes) and Rab5 (EE). (C) EEA1 (EE) and Rab7 (LE). (D and E) EEA1 (EE) and phalloidin (filamentous actin). Arrows mark engulfed latex beads within the cytoplasm. (Scale bar, 10 µm.) EE, early endosomes; LE, late endosomes; TGN, trans Golgi network.